200752000 - Genetic Predisposition of Smoltification in Rainbow Trout and Steelhead

Sponsor: Columbia River Inter-Tribal Fish Commission

Short description: This proposal seeks to identify quantitative genetic characteristics that predispose individual O. mykiss to smoltification. We will investigate the potential of resident rainbow trout to contribute to the recovery of ESA listed steelhead populations.

Location: Columbia Gorge province, Klickitat subbasin

Budgets: FY08: $44,837 | FY09: $45,141

Primary species:
Anadromous: Steelhead: Middle Columbia River ESU (threatened)
 Resident: Rainbow Trout

Objectives

ObjectiveDescriptionSubbasin planStrategies
1. Identify smoltification genes This objective focuses on the use of quantitative genetic markers to evaluate the potential of resident rainbow trout to contribute to recovery of steelhead populations. Both environmental and genetic factors determine if individual O. mykiss remain as resident rainbow trout, or undergo the necessary physiological changes (smoltification) to prepare for anadromy. While some of the associated environmental factors (i.e., water flow and temperature) have been evaluated, the genetic mechanisms that contribute to life history selection are unknown. This proposal seeks to identify quantitative genetic characteristics that predispose individual O. mykiss to smoltification. The study focuses on three tributaries to the Yakima River with varying abundance of each life history type: 1) White Creek - near exclusive steelhead population, 2) upper Summit Creek - predominant resident population, and 3) Snyder Creek - mixed population with both life history types. The study will utilize quantitative genetics technology such as 5’ endonuclease assays (TaqMan) and microarrays to identify genes that regulate expression of smoltification in O. mykiss. This objective will identify genetic traits that could be used to recover threatened steelhead populations. Klickitat Restoring steelhead populations in the Klickitat River is one of the primary goals and biological objectives in the subbasin plan. Strategies in this sub-basin include restoring steelhead above previous barriers. The proposal also pertains to mainstem.

Narrative

Recommendations

  • ISRP: Unranked
  • Council: Not fundable
  • BPA: Not fundable